The N-terminal region of severe acute respiratory syndrome coronavirus protein 6 induces membrane rearrangement and enhances virus replication.
Identifieur interne : 002427 ( Main/Exploration ); précédent : 002426; suivant : 002428The N-terminal region of severe acute respiratory syndrome coronavirus protein 6 induces membrane rearrangement and enhances virus replication.
Auteurs : Haixia Zhou [États-Unis] ; Debra Ferraro ; Jincun Zhao ; Snawar Hussain ; Jianqiang Shao ; Jonathan Trujillo ; Jason Netland ; Thomas Gallagher ; Stanley PerlmanSource :
- Journal of virology [ 1098-5514 ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Viral Proteins.
- chemical , metabolism : STAT1 Transcription Factor.
- chemistry : Cell Membrane.
- physiology : SARS Virus, Viral Proteins.
- Active Transport, Cell Nucleus, Cells, Cultured, Humans, Protein Structure, Secondary, Protein Transport, Virus Replication.
Abstract
The severe acute respiratory syndrome coronavirus (SARS-CoV) accessory protein 6 (p6) is a 63-amino-acid multifunctional Golgi-endoplasmic reticulum (ER) membrane-associated protein, with roles in enhancing virus replication and in evading the innate immune response to infection by inhibiting STAT1 (signal transducer and activator of transcription factor 1) translocation to the nucleus. Here, we demonstrate that p6 has an N-terminal region-cytoplasm-C-terminal region-cytoplasm configuration with residues 2 to 37 likely membrane embedded. Expression of p6, or of its N-terminal 41-amino-acid region, in the absence of other viral proteins, induced the formation of membranous structures, some of which were similar to double membrane vesicles involved in virus replication. Consistent with a role in virus replication, p6 partially colocalized with nonstructural protein 3 (nsp3), a marker for virus replication complexes. Further, while the C-terminal region is required for preventing STAT1 translocation to the nucleus, our results also indicated that the N-terminal 18 amino acids were necessary for maximal inhibition. Collectively, these results support the notion that p6 is a two-domain protein, although the function of each is not completely independent of the other.
DOI: 10.1128/JVI.02570-09
PubMed: 20106914
Affiliations:
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Le document en format XML
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<term>Protein Transport</term>
<term>SARS Virus (physiology)</term>
<term>STAT1 Transcription Factor (metabolism)</term>
<term>Viral Proteins (chemistry)</term>
<term>Viral Proteins (physiology)</term>
<term>Virus Replication</term>
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<term>Protéines virales ()</term>
<term>Protéines virales (physiologie)</term>
<term>Réplication virale</term>
<term>Structure secondaire des protéines</term>
<term>Transport de protéines</term>
<term>Transport nucléaire actif</term>
<term>Virus du SRAS (physiologie)</term>
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<term>Viral Proteins</term>
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<front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus (SARS-CoV) accessory protein 6 (p6) is a 63-amino-acid multifunctional Golgi-endoplasmic reticulum (ER) membrane-associated protein, with roles in enhancing virus replication and in evading the innate immune response to infection by inhibiting STAT1 (signal transducer and activator of transcription factor 1) translocation to the nucleus. Here, we demonstrate that p6 has an N-terminal region-cytoplasm-C-terminal region-cytoplasm configuration with residues 2 to 37 likely membrane embedded. Expression of p6, or of its N-terminal 41-amino-acid region, in the absence of other viral proteins, induced the formation of membranous structures, some of which were similar to double membrane vesicles involved in virus replication. Consistent with a role in virus replication, p6 partially colocalized with nonstructural protein 3 (nsp3), a marker for virus replication complexes. Further, while the C-terminal region is required for preventing STAT1 translocation to the nucleus, our results also indicated that the N-terminal 18 amino acids were necessary for maximal inhibition. Collectively, these results support the notion that p6 is a two-domain protein, although the function of each is not completely independent of the other.</div>
</front>
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<name sortKey="Gallagher, Thomas" sort="Gallagher, Thomas" uniqKey="Gallagher T" first="Thomas" last="Gallagher">Thomas Gallagher</name>
<name sortKey="Hussain, Snawar" sort="Hussain, Snawar" uniqKey="Hussain S" first="Snawar" last="Hussain">Snawar Hussain</name>
<name sortKey="Netland, Jason" sort="Netland, Jason" uniqKey="Netland J" first="Jason" last="Netland">Jason Netland</name>
<name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
<name sortKey="Shao, Jianqiang" sort="Shao, Jianqiang" uniqKey="Shao J" first="Jianqiang" last="Shao">Jianqiang Shao</name>
<name sortKey="Trujillo, Jonathan" sort="Trujillo, Jonathan" uniqKey="Trujillo J" first="Jonathan" last="Trujillo">Jonathan Trujillo</name>
<name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name>
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<country name="États-Unis"><region name="Iowa"><name sortKey="Zhou, Haixia" sort="Zhou, Haixia" uniqKey="Zhou H" first="Haixia" last="Zhou">Haixia Zhou</name>
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